who uses the emkaPACK non-invasive telemetry systems and how

Emily Bailey, Claire Grant, Claire Barnard and Pierre Lainee, AstraZeneca, Global Safety Assessment, Alderley Park, Macclesfield, United Kingdom, 2011

inclusion of blood pressure measurements in canine toxicology studies

Validation of a non-invasive telemetry method

• blood pressure recordings up to 24 hours
• bp, ecg and respiratory parameters recorded simultaneously
• 40s-1min to perform a cycle (inflation and deflation)

poster presented by GlaxoSmithKline, Ware, UK, Safety Pharmacology and Statistical Data Sciences at the Society of Toxicology meeting, Washington, DC, March 2011

assessment of non-invasive telemetric blood pressure and electrocardiogram measurement in conscious dogs

evaluation of the veracity of a noninvasive telemetry system for detecting changes in blood pressure, heart rate and electrocardiogram in ambulatory dogs

• male beagle dogs with tail cuffs
• BP (mean, systolic, diastolic and pulse), HR, RR, PR, QRS, QT and QTcL

presention made by Aidan K. Curran, HLS, East Millstone, NJ, USA at the Safety Pharmacology Society meeting 2010, Boston, MA, USA, Septemeber 2010

the use of respiratory inductance plethysmography for non-invasive evaluation of pulmonary function in dogs and monkeys

RIP allows for significantly more data in freely moving animals over the entire period of drug exposure. overview:

• physiology of breathing
• inductance plethysmography theory
• the theory
• the practice
• specific parameters
• ventilation
• phase angle (pulmonary resistance)
• discussion
• benefits and drawbacks

Cécile Fligny,* Yves Fromes,† Philippe Bonnin,§ Michèle Darmon,‡ Elisa Bayard,* Jean-Marie Launay,|| Francine Côté,* Jacques Mallet,*,1 and Guilan Vodjdani*,1, The FASEB Journal, Vol. 22 July 2008, *Unité Mixte de Recherche 7091 Centre National de la Recherche Scientifique/Université Pierre et Marie Curie, Laboratoire de Génétique Moléculaire de la Neurotransmission et des Processus Neurodégénératifs (LGN), †Institut de Myologie, U582 Institut National de la Santé et de la Recherche Médicale, and ‡Unité Mixte de Recherche 677 Institut National de la Santé et de la Recherche Médicale/ Université Pierre et Marie Curie, Laboratoire de Neuropsychopharmacologie, Hôpital de la Pitié-Salpêtrière, Paris, France; and § Centre de Recherche Cardiovasculaire, Physiologic Explorations Fonctionnelles, U689 Institut National de la Santé et de la Recherche Médicale, Université Denis Diderot, and ||Service de Biochimie, Hôpital Lariboisière, APHP, Paris, France

serotonin influences cardiac function in adult offspring

cardiac function, through the plasma 5-HT concentration, is influenced by the maternal serotonergic status

• maternal effect
• heart failure animal model
• dilated cardiomyopathy
• ecgTUNNEL restraining device for small rodents
• emkaPACK external telemetry

poster presented by AstraZeneca, Global Safety Assessment, Alderley Park, Macclesfield, United Kingdom at the Society of Toxicology meeting, Salt Lake City, UT, March 2010

ecg recording methods in dog toxicology studies: what is the real benefit of external telemetry?

The availability of external telemetry using jacketed animals offers an interesting alternative that has been assessed in Tox studies for more than five years now. This review presents a comparison of historical data obtained using three different methods. Comparison of:

• standard snapshot recordings
• external telemetry (emka TECHNOLOGIES)
• standard invasive telemetry

poster presented by CIT at the Safety Pharmacology Society meeting, Strasbourg, France, September 2009

non-invasive blood pressure monitoring in ambulatory beagle dogs

it is currently possible to record ecg, respiration and behavior in freely moving non-rodent models by non-invasive external telemetry associated with video recording

• feasibility and effi cacy of a novel technique for non-invasive blood pressure (nibp) recording by telemetry in dogs
• oscillometric cuff method developed by emka TECHNOLOGIES
• a cross-validation test was performed to compare concomitant recordings of ABP signals obtained with an implanted catheter (internal telemetry system) and with the NIBP system (external telemetry system).

poster presented by Charles River Laboratories, Edinburgh, UK, at the Society of Safety Pharmacology meeting, Edinburgh, UK, September 2007

use of the emka TECHNOLOGIES system for ecg assessments on toxicology studies

study performed on beagle dogs - feasibility study of automating the capture and analysis of ecgs on toxicology studies using the emka data acquisition/analysis system

• traditional methods of paper traces versus automated analysis
• fda 21 cfr part 11 compliant system
• screen captures of ecgAUTO with 6 lead ecg and ecg analysis

poster presented by Roche Basel, Switzerland, at the Society of Toxicology meeting, Baltimore, MD, March 2009

sensitivity of telemetry to predict QT interval prolongation in dog toxicity studies: assessment of the effects of moxifloxacin by invasive and non-invasive methods

study performed on dogs to determine each system's sensitivity in detecting QT interval prolongation using moxifloxacin as a positive agent

• screen captures of signal acquired from emka TECHNOLOGIES and from ITS.
• photos of electrode placement, and dog jacket
• jacket telemetry fulfills the 3R criteria

poster presented by Roche Palo Alto, CA, USA, at the Safety Pharmacology Society meeting, Madison, WI, September 2008

comparisons of emka TECHNOLOGIES jacket system and DSI™ implant in conscious dogs with moxifloxacin

study performed on dogs to determine each system's sensitivity in detecting QTc prolongation using moxifloxacin as a positive agent

• screen captures of same signal on emka TECHNOLOGIES and on DSI™.

poster presented by Pfizer Ann Arbor
2003

improved methods for electrocardiogram monitoring in ambulatory dogs

study performed on dogs

• explains how to better equip animals and make best use of the emka TECHNOLOGIES non-invasive telemetry system
• photo of sleeping dog with jacket

poster shown by the CIT Evreux, France, at the Safety Pharmacology Society meeting, Mannheim, Germany, September 2005

external telemetry allows investigation of dose and time-related effects on ecg parameters within toxicity studies

studies performed on dogs and monkeys

• Shows 6 lead ecg traces from system
• QT prolongation trend graph in primate
• arrhythmias detection
• group housing

presentation by the CIT Evreux, France, at the Safety Pharmacology Society meeting, San Diego, CA, September 2006

external telemetry : a breath of fresh air - respiratory parameters are now available and can improve the core battery investigations

studies performed on dogs and monkeys - respiration measurement

• photos of dogs and primates equipped with jackets
• photos of dogs being equipped with respiration belt, respiration calibration
• photos of receiving station on trolley and antenna on tripod

poster presented by AstraZeneca Macclesfield, UK, at Safety Pharmacology Society meeting, 2003

comparison of non-invasive versus invasive telemetry

study performed on 6 male beagle dogs

• data produced is comparable to that obtained from implanted DSI™ system
• how to equip animals
• 24hr ecg recording is an improvement over traditional “snap shot” (1mn) recordings
• scatter graphs of QT, PR, HR data (DSI™ and emka TECHNOLOGIES

poster presented by AstraZeneca Macclesfield, UK, at Safety Pharmacology Society meeting, 2003

use of non-invasive telemetry system (emka TECHNOLOGIES) for cardiovascular endpoints in toxicology studies

27 day study performed on 2 groups of 6 dogs - 5 24-hour measurement points

• comparison of effects of vehicle and compound
• comparison of advantages and disadvantages of method (mentions that only 1 lead is available: it is now possible to go up to 6 standard lead + chest lead)
• photos of dogs
• screencapture of ecgAUTO analysis report screen

poster presented by
AstraZeneca Macclesfield, UK,
at the Society of Toxicology annual meeting, San Diego, CA, March 2006

optimisation of a non-invasive telemetry system for electrocardiogram assessment in dogs: a 3-years review

studies performed on beagle dogs - 3 years of experience with emka TECHNOLOGIES ecg telemetry hardware and software - 17 studies, 10,000 hours of data, 60 million ecg complexes

• dog jackets
• acclimatisation routine
• library of ecg waveforms for ecgAUTO software
• comparison with results acquired from subjects implanted with DSI telemetry
• comparison with results acquired from subjects restrained in a sling

poster presented by
AstraZeneca Macclesfield, UK,
at the Society of Toxicology annual meeting, San Diego, CA, March 2006

application of a non-invasive telemetry system (emka) for electrocardiogram assessment in a dog toxicology study

study performed on beagle dogs

• emka TECHNOLOGIES telemetry system is GLP compliant
• loss of data was 3.6%

M. Zawada and Lewis B. Kinter
AstraZeneca, USA
2005

obtaining quality ecg endpoints in toxicology studies : is restraint necessary ?

compares fully implanted, partially implanted and non-implanted systems

• electrode placement on dogs
• photos of receiver hardware on trolley with antenna on tripod
• photos of dogs in pens
• screencaptures of ecgAUTO ecg analysis software

animal technology and welfare, K. Moyser, J. Schofield, D. Simpson, D. Hunter, AstraZeneca UK
April 2004

benefits and limitations of an external telemetry system on canine toxicity studies

study performed on dogs to determine each system's sensitivity in detecting QTc prolongation using moxifloxacin as a positive agent